New microsatellite markers developed from an enriched microsatellite common bean library
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چکیده
منابع مشابه
Microsatellite markers isolated from the wild medicinal plant Centella asiatica (Apiaceae) from an enriched genomic library.
PREMISE OF THE STUDY Microsatellite markers for Centella asiatica, an important medicinal herb, were developed and characterized to promote genetic and molecular studies. METHODS AND RESULTS A GA/GT-enriched genomic library was constructed from an accession from Madagascar. Roughly 75% of the 768 clones of the enriched library contained microsatellites. Eighty sequences containing microsatell...
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The objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virus and, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed. For crosses ARC100-4 x DOR303 a...
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PREMISE OF THE STUDY Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. METHODS AND RESULTS Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loc...
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Analysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the be...
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PREMISE OF THE STUDY Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. METHODS AND RESULTS Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome ass...
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ژورنال
عنوان ژورنال: Pesquisa Agropecuária Brasileira
سال: 2008
ISSN: 0100-204X
DOI: 10.1590/s0100-204x2008000700019